Quantitative Detection of Respiratory Chlamydia pneumoniae Infection by Real-Time PCR
نویسندگان
چکیده
منابع مشابه
Quantitative detection of respiratory Chlamydia pneumoniae infection by real-time PCR.
Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection.
متن کاملSIMULTANEOUS DETECTION OF CHLAMYDIA PNEUlvlONIAE AND MYCOPLASMA PNEUMONIAE BY PCR
Both C. pneumoniae and M. pneumoniae are common causes of respiratory tract infection. At present, both are still diagnosed in the laboratory retrospectively by serology. This is despite many publications which indicate that PCR, which is not retrospective, is extremely good at detecting these organisms. We thought that a single PCR test which could detect both organisms simultaneously in a...
متن کاملDevelopment of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملQuantitative detection of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in lower respiratory tract samples by real-time PCR.
The limitation of polymerase chain reaction (PCR) in diagnosis of lower respiratory tract infections (LRTIs) caused by Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis has been a distinguishing colonization from infection. We assess here the usefulness of real-time quantitative PCR (RQ-PCR) performed on lower respiratory tract samples to overcome this problem. Consecu...
متن کاملQuantitative detection of Streptococcus pneumoniae in nasopharyngeal secretions by real-time PCR.
Streptococcus pneumoniae is an important cause of community-acquired pneumonia. However, in this setting the diagnostic sensitivity of blood cultures is below 30%. Since during such infections changes in the amounts of S. pneumoniae may also occur in the upper respiratory tract, quantification of these bacteria in nasopharnygeal secretions (NPSs) may offer a suitable diagnostic approach. Real-t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2002
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.40.6.2273-2274.2002